permissive hek 293 host cell line Search Results


hek 293 atcc crl 1573 cos 7 atcc crl 1651 u266 atcc tib 196 huns1 atcc crl 8644 chl ecacc no 87111906 host cells  (ATCC)
99
ATCC hek 293 atcc crl 1573 cos 7 atcc crl 1651 u266 atcc tib 196 huns1 atcc crl 8644 chl ecacc no 87111906 host cells
Hek 293 Atcc Crl 1573 Cos 7 Atcc Crl 1651 U266 Atcc Tib 196 Huns1 Atcc Crl 8644 Chl Ecacc No 87111906 Host Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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fetal bovine serum fbs human embryonic kidney hek 293t cells host animal  (Sartorius AG)
98
Sartorius AG fetal bovine serum fbs human embryonic kidney hek 293t cells host animal
Fetal Bovine Serum Fbs Human Embryonic Kidney Hek 293t Cells Host Animal, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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viral host protein protein interaction assay 293t cells  (Greiner Bio)
99
Greiner Bio viral host protein protein interaction assay 293t cells
Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. <t>293T</t> cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.
Viral Host Protein Protein Interaction Assay 293t Cells, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human embryonic kidney 293 host cell proteins elisa kit  (Cygnus Technologies)
90
Cygnus Technologies human embryonic kidney 293 host cell proteins elisa kit
Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. <t>293T</t> cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.
Human Embryonic Kidney 293 Host Cell Proteins Elisa Kit, supplied by Cygnus Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek 293 host cell proteins elisa kit  (Cygnus Technologies)
90
Cygnus Technologies hek 293 host cell proteins elisa kit
Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. <t>293T</t> cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.
Hek 293 Host Cell Proteins Elisa Kit, supplied by Cygnus Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek r4 cell host  (Thermo Fisher)
86
Thermo Fisher hek r4 cell host
Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. <t>293T</t> cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.
Hek R4 Cell Host, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek r4 cell host - by Bioz Stars, 2026-03
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hek-293-flp-in t-rex host cells  (Thermo Fisher)
90
Thermo Fisher hek-293-flp-in t-rex host cells
Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. <t>293T</t> cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.
Hek 293 Flp In T Rex Host Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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host cell line hek293t  (TaKaRa)
94
TaKaRa host cell line hek293t
Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. <t>293T</t> cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.
Host Cell Line Hek293t, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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permissive hek 293 host cell line  (Bio-Rad)
93
Bio-Rad permissive hek 293 host cell line
Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. <t>293T</t> cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.
Permissive Hek 293 Host Cell Line, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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host cell 293t cell  (ATCC)
99
ATCC host cell 293t cell
Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. <t>293T</t> cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.
Host Cell 293t Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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host cell 293t cell - by Bioz Stars, 2026-03
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2 material htl-1  (Becton Dickinson)
90
Becton Dickinson 2 material htl-1
Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. <t>293T</t> cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.
2 Material Htl 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. 293T cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Exploration of Binary Virus–Host Interactions Using an Infectious Protein Complementation Assay *

doi: 10.1074/mcp.M113.028688

Figure Lengend Snippet: Identification of cellular interactors of influenza virus polymerase via iPCA. A, schematic of the experiment. 293T cells seeded in 96-well plates were transfected with an ordered set of 84 plasmids encoding human proteins fused to Gluc2 (Gluc2-human P), or with control plasmids. At 18 h post-transfection, 293T cells were infected at a high multiplicity of infection (>1 pfu/cell) with an influenza virus expressing a Gluc1-tagged polymerase (viral P-Gluc1) or with the wild-type control virus. At 6 h post-infection, cell lysates were prepared and normalized luminescence ratios (NLRs) were determined by dividing the luminescence activity measured in cells co-expressing a Gluc2-human P and a viral P-Gluc1 by the sum of the luminescence activities measured in the corresponding control samples. B, Gaussian curves fitted on log(NLR) values of the random reference set (RRS) and the literature-curated set (LCS) with the PB2-Gluc1, PB1-Gluc1, and PA-Gluc1 viruses. The positive threshold value of 4 is indicated. C, heat map of the NLRs. The 84 screened cellular proteins are arranged by hierarchical clustering according to their NLRs. The 58 proteins that belong to the LCS and the 26 proteins that belong to the exploratory set (Exp) are indicated. The color scale indicates the range of NLRs measured upon screening with PB2-Gluc1, PB1-Gluc1, or PA-Gluc1 recombinant influenza virus.

Article Snippet: Viral–Host Protein–Protein Interaction Assay 293T cells were seeded at a concentration of 3 × 10 4 cells per well in 96-well white plates (Greiner Bio-One, Courtaboeuf, France).

Techniques: Transfection, Infection, Expressing, Activity Assay, Recombinant